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LifeLines Cohort Study & Biobank [LifeLines] Download as PDF

GENERAL INFORMATION

Name LifeLines Cohort Study & Biobank
Acronym LifeLines
Last update 03/23/2009

Affiliation

Principal investigator

Bruce HR Wolffenbuttel (Scientific Director)
University Medical Centre Groningen

Contact

Bruce HR Wolffenbuttel (Scientific Director)
University Medical Center Groningen
Groningen P.O. Box 30001
Phone: +31-50-3613962
Email: bwo@int.umcg.nlMail

Website

Funding agency

Percentage Category
Dutch Diabetes Research Foundation -- Charity
Governement, 3 northern provinces of The Netherlands 35% Governmental funding
University Med Center Groningen/ University of Groningen 44% --
The Netherlands Organization for Scientific Research (NWO) 16% Public Research Funding
Dutch Kidney Foundation -- Charity

BACKGROUND

Multifactorial diseases are by definition the result of multiple risk factors that are both genetically and environmentally determined. Multifactorial diseases may have more in common than generally recognized, since some risk factors are associated with multiple diseases, suggesting an individual susceptibility for specific risk factors.
Since different diseases share identical risk factors, it is clear that an exclusive focus on associations between single risk factors and single outcomes will not unravel the unresolved issues of aetiology and individual prognosis of multifactorial diseases. Instead, research has to focus on the underlying mechanisms why individuals with similar (established) risk factors develop different diseases, i.e. the modification of the universal risk factors for multiple disorders.

OBJECTIVES

Overall aim of the study:
Unravel the interaction between genetic and environmental factors in the development of multifactorial diseases, their concurrent development in individuals and their complications as a complex trait.
Primary objectives:
Which are the disease overriding risk factors which predict the development of a multifactorial disease during lifetime? How are these universal risk factors modified, or what determines the effect of a universal risk factor in an individual? Specific research questions will focus on risk factors and modifiers (genetic, environmental and combined or complex factors) for single and multiple diseases. In addition to co-morbidity, LifeLines focuses on co-determinants. The primary endpoints include measures of ageing, metabolic and endocrine diseases, cardiology, renal diseases, pulmonary and musculoskeletal diseases, and psychopathology.
Secondary objectives:
Secondary aims include the assessment of the prevalence and incidence of multifactorial diseases and their risk factors in individuals as well as in families. The burden of disease for the society will be quantified in terms of care needed, and total costs of care.

Topics of interest

  • No Broad approach with no specific topic of interest
  • Yes Target specific topics of interest (health outcomes, health determinants, etc.)
Topics ICD-10 classificationExternal
Yes Endocrine, nutritional and metabolic diseases (E00-E90)
Yes Mental and behavioural disorders (F00-F99)
Yes Diseases of the circulatory system (I00-I99)
Yes Diseases of the respiratory system (J00-J99)
Yes Diseases of the digestive system (K00-K93)
Yes Diseases of the skin and subcutaneous tissue (L00-L99)
Yes Diseases of the musculoskeletal system and connective tissue (M00-M99)
Yes Diseases of the genitourinary system (N00-N99)

Supplementary informations

General focus on frequently occurring multifactorial disorders and multidisease factors

METHODS

Legend Yes No Unknown

General design

Study design Cohort
Type of participants Individuals, Families
Supplementary informations The study population consists of a representative sample of the population of the northern provinces of the Netherlands (Friesland, Groningen and Drenthe), existing of 165,000 persons in three generations. These include 55,000 in the age group > 50 yrs, 75,000 in the age group between 25 and 50, and 35,000 in the age group below 25 yrs.
Target or final number of participants 165000
  The project will include 165.000 participants: 30.000 probands, 20.000 partners, 75.000 parents (in law) and 40.000 children.
    Target or final number of families 30000
Target or final number of DNA 165000

Participant selection / Characteristics of the population

Selection criteria

Yes Age Minimun: 25 years    Maximun: 50 years
Yes Country of residence Netherlands (Three Northern provinces: Groningen, Friesland and Drenthe)
Yes Health status / clinical criteria (Severe psychiatric or physical illness are excluded)
Yes Other criteria Being able to speak Dutch.

Recruitment procedures

The study population consists of a representative sample of the population of the northern provinces of the Netherlands (Friesland, Groningen and Drenthe), existing of 165,000 persons.
Persons registered in a general practitioner’s practice in Groningen, Friesland and Drenthe, between 25 and 50 years of age. After inclusion of the proband, the partners, parents, parents in law and children of these participants will be asked to participate in the study.

The recruitment of participants will be carried out in a selected number of medical practices. All patients in a medical practice, in the age range of 25-50 years, will be asked to join the study. Via information obtained from the medical practice, individuals will be asked by mail to participate in the cohort study. In the Netherlands, the average general practice size is around 2,000-2,400 patients, although combined or group practices may be larger. In the pilot study, it was learned that about 35 - 40% of the eligible population from each practice will participate. It is estimated that around 135,000 individuals in the age category of 25-50 years will need to be approached to provide the required sample of 45,000 index participants.
The participating practices will be selected from interested rural and municipal practices within the three Northern provinces, and care is taken that they represent all socioeconomic layers of the population. They are considered eligible to take part in the study if they have an interest in research and use computerised patient and prescription records. Over 80% of practices in The Netherlands use such computer programs. Practices will be selected so as to cover a broad range of socio-economic conditions. In case selected practices have insufficient space for performing the LifeLines related activities, the evaluation of participants and their follow-up may be organized through municipal health centres or corresponding facilities (dedicated Research sites) in the vicinity of the G.P. practice(s), which will be set up through the University Medical Centre Groningen.

Data Sources

  Cross-sectional Repeated/continuous
Yes Questionnaires to participants/respondents Yes Yes
Yes Direct physical measures Yes Yes
Yes Biological samples Yes Yes
Yes Medical paper
Yes Electronic databases
No Genealogical records  

Collection procedures

Intake visits to General Practitioner or Research Site
During the first visit the signed informed consent is taken in. If requested, additional information will be provided.
During the first and second visit, the first or second part of the questionnaire, respectively, are taken in and checked for completeness, a number of investigations are conducted and blood and urine samples are taken (see below). The second visit is scheduled approximately 2 weeks after the first visit, depending on available time and preference of the participant.
For participants of 65 years and older, a MMSE (minimal mental state examination) will be conducted in order to determine the capabilities of the participant. An adapted program or visits at home by the research nurse will be conducted; a decision to do so is at the discretion of the participating general practitioner. In principle, the adapted program will identical to the standard program instead that the questionnaires will be divided in several parts. Additionally, for all participants, investigations will not be performed when the participant is physically or mentally not capable in the performance of one or more specific investigations, a decision to do so is at the discretion of the research nurse.

The participant is asked to fill the questionnaire in at home (on paper or online), and to return the paper versions by mail or to deliver it at the first scheduled visit. If there are problems filling in the questionnaire, LifeLines personnel can be contacted for assistance. The baseline questionnaire comprises Demographics, Family composition, Work and educational level, Health, Questions for females, Birth and development, Lifestyle and diet, Day time spending, Living environment, Quality of life, Health perception, Personality, Stress and Social support
Measurements comprise amongst others Anthropometric measures: height, weight, hip and waist circumference, Blood pressure: using a DINAMAP, measurements during 10 minutes, 12-lead electrocardiogram (ECG), Pulmonary function tests (FEV1 and VC), Ruffs Figure Fluency Test (RFFT): test of cognitive function, MINI interview: neuropsychiatric interview in which a number of basic questions are asked about fears and depression.
Second visit comprises Fasting venapuncture, Measurement of skin autofluorescence with a non-invasive AGE-reader (Advanced Glycation Endproducts), 24 hour urine, First morning urine.

Follow up procedures

Yearly, the participants will receive a questionnaire, consisting of incidence of diseases / visits to health care providers, Medication use, Additional information on health determinants, e.g. nutrition.

Every 4-5 years participants are invited for a visit to the general practitioner or research site. These follow-up visits include blood sampling, urine collection and measurements like the baseline measurements. A questionnaire comparable to the baseline questionnaire is used. Maximal efforts will be used to have follow-up examinations in most participants.

Baseline principal variables of interest

Health information

  • Yes Diseases history - ICD-10
  • Yes Medication intake
  • Yes Familial disease history
  • Yes Early life
  • Yes Women's health
  • Yes Quality of life

Physical / Biochemical measures

Yes Body structure measures Yes Biochemical measures
Yes Body function measures  

Sociodemographic Characteristics

Yes Birth location Yes Parents birth location
Yes Ethnicity/Race Yes Gender
Yes Marital status Yes Date of birth

Socioeconomic Characteristics

Yes Education level Yes Income
Yes Working status  

Life habits / Behaviours

Yes Physical activity Yes Smoking / Tobacco Use
Yes Nutrition Yes Alcohol intake

Physical environment

Yes Passive smoking Yes Mobile phone use

Sample management

Biological samples

Yes Blood No Buccal cells
No Cord blood No Tissues
No Saliva Yes Urine

Biological samples format

  Fresh Frozen Dried (paper) Other Specification
Blood Yes Yes Yes No
Cell lines No Yes   No

Genome-Wide Association (GWA) analyses

  • Yes Plan to proceed to GWA analyses later

GOVERNANCE

Legend Yes No

Allow access to data or samples to external researchers

Academia Industry
Yes Data (questionnaire-derived, measured…) Yes Yes
Yes Biological samples Yes

STATUS

Current status

Phase Start End
Preparation phase/Pilot 2004 2006
Recruitment / initial data collection 2007 2011
Follow-up of participants 2012 2015
Study ended   2036
Current number of participants recruited 20000 in date of 05/01/2008
Current number of collected DNA samples 20000 in date of 05/01/2008
Participation rate 35%

AVAILABLE DOCUMENTS

  Document type Availability
1 General study protocol bwo@int.umcg.nlMail
2 Specific protocols/procedures (data collection, samples management, etc.) bwo@int.umcg.nlMail
3 Questionnaires bwo@int.umcg.nlMail
4 Physical and cognitive measures
5 DNA processing
6 Consent forms bwo@int.umcg.nlMail
7 Pamphlet / project description bwo@int.umcg.nlMail
8 Governance / ethics documentation
9 Other

PUBLICATIONS AND RELEVANT DOCUMENTATION

1
Stolk Universal risk factors for multifactorial diseases: LifeLines: a three-generation population-based study European Journal of Epidemiology 2008 67-74

COMMENT

Funding for baseline measurement and partly for follow-up has been obtained


General Information

Name LifeLines Questionnaire
Last update 01/06/2010
Phase Baseline

Contact

No information available. See study information.

Documents

Available Format

NOTICE: HTML document format may differ slightly from the actual questionnaire. Please refer to the original PDF document for exact reproduction.

General Information

Name LifeLines Questionnaire
Number of Question Blocks 62

Questionnaire and Question Block List

Click on the View block to see the block of questions related to a domain or a group of domains.
Block 2 View block
  • Sociodemographic Characteristics / Gender
Block 3 View block
  • Sociodemographic Characteristics / Age/Birth Date / Subject age / birth date
Block 4 View block
  • Sociodemographic Characteristics / Birth location / Subject's birth location
Block 5 View block
  • Sociodemographic Characteristics / Birth location / Subject's family's birth location
Block 6 View block
  • Sociodemographic Characteristics / Household Status / Subjet Household Status
Block 7 View block
  • Social Environment / Familial and social environment
  • Sociodemographic Characteristics / Residence / Residential History
Block 8 View block
  • Sociodemographic Characteristics / Household Status / Structure of the Household
Block 9 View block
  • Body Functions / Handedness / laterality / Handedness
Block 10 View block
  • Sociodemographic Characteristics / Age/Birth Date / Subject's Relatives Age / Birth Date
Block 11 View block
  • Socioeconomic Characteristics / Working status / Subject Working status/history
Block 12 View block
  • Socioeconomic Characteristics / Income
Block 13 View block
  • Socioeconomic Characteristics / Working status / Subject Working status/history
Block 14 View block
  • Socioeconomic Characteristics / Education level / Subject education level
Block 15 View block
  • Socioeconomic Characteristics / Working status / Subject Working status/history
Block 16 View block
  • Social Environment / Familial and social environment
Block 17 View block
  • Life Habits/Behaviours / Sleep Behaviours
Block 18 View block
  • Health Information / Individual Disease history / Diseases of the respiratory system
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the circulatory and respiratory systems
Block 19 View block
  • Health Information / Individual Disease history / Diseases of the respiratory system
Block 20 View block
  • Health Information / Individual Disease history / Factors influencing health status and contact with health services
  • Health Information / Individual Disease history / Injury, poisoning and certain other consequences of external causes
Block 21 View block
  • Health Information / Individual Disease history / Neoplasms
Block 22 View block
  • Health Information / Individual Disease history / Endocrine, nutritional and metabolic diseases
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
Block 23 View block
  • Health Information / Individual Disease history / Diseases of the circulatory system
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Cardiovascular System
  • Health Information / Medication Intake (ATC/DDD) / Cardiovascular system (C)
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the circulatory and respiratory systems
Block 24 View block
  • Health Information / Individual Disease history / Endocrine, nutritional and metabolic diseases
Block 25 View block
  • Health Information / Individual Disease history / Diseases of the circulatory system
  • Health Information / Individual Disease history / Diseases of the nervous system
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving speech and voice
Block 26 View block
  • Health Information / Individual Disease history / Diseases of the musculoskeletal system and connective tissue
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Musculoskeletal System
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
Block 27 View block
  • Health Information / Individual Disease history / Diseases of the genitourinary system
Block 28 View block
  • Health Information / Medical Health Intervention (C239) / Diagnostic Interventions / Diagnostic Interventions on the Genitourinary System
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Abnormal findings on examination of urine, without diagnosis
Block 29 View block
  • Health Information / Individual Disease history / Diseases of the genitourinary system
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Genitourinary System
Block 30 View block
  • Health Information / Individual Disease history / Endocrine, nutritional and metabolic diseases
Block 31 View block
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Eyes and Ocular Adnexa
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
Block 32 View block
  • Health Information / Medical Health Intervention (C239) / Cognitive, Psychosocial and Sensory Therapeutic Interventions / Therapeutic Interventions for Hearing
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
Block 33 View block
  • Health Information / Individual Disease history / Diseases of the digestive system
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Orocraniofacial Unit
Block 34 View block
  • Health Information / Individual Disease history / Diseases of the blood and blood-forming organs and certain disorders involving the immune mechanism
  • Health Information / Individual Disease history / Diseases of the circulatory system
  • Health Information / Individual Disease history / Diseases of the digestive system
  • Health Information / Individual Disease history / Diseases of the eye and adnexa
  • Health Information / Individual Disease history / Diseases of the musculoskeletal system and connective tissue
  • Health Information / Individual Disease history / Diseases of the skin and subcutaneous tissue
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Individual Disease history
  • Health Information / Individual Disease history / Diseases of the genitourinary system
  • Health Information / Individual Disease history / Diseases of the nervous system
  • Health Information / Individual Disease history / Diseases of the respiratory system
Block 36 View block
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Respiratory System
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Cardiovascular System
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Digestive and Hepatobiliary Tract
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Genitourinary System
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Musculoskeletal System
Block 37 View block
  • Health Information / Medication Intake (ATC/DDD) / Blood and blood forming organs (B)
  • Health Information / Medication Intake (ATC/DDD) / Musculo-skeletal system (M)
  • Health Information / Medication Intake (ATC/DDD) / Nervous system (N)
  • Health Information / Medication Intake (ATC/DDD) / Various
  • Health Information / Medication Intake (ATC/DDD)
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
  • Health Information / Medication Intake (ATC/DDD) / Cardiovascular system (C)
Block 38 View block
  • Health Information / Medical Health Intervention (C239) / Cognitive, Psychosocial and Sensory Therapeutic Interventions / Therapeutic Interventions for Communication
  • Health Information / Medical Health Intervention (C239) / Cognitive, Psychosocial and Sensory Therapeutic Interventions / Therapeutic Interventions for Voice
  • Health Information / Medical Health Intervention (C239) / Cognitive, Psychosocial and Sensory Therapeutic Interventions / Therapeutic Interventions on the Psyche (mental health)
  • Health Information / Medical Health Intervention (C239) / Other Healthcare Interventions
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Musculoskeletal System
Block 39 View block
  • Health Information / Perception of health/quality of life
Block 40 View block
  • Life Habits/Behaviours / Physical activity / Perception of physical health
Block 41 View block
  • Health Information / Perception of health/quality of life
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
  • Social Environment / Familial and social environment
Block 42 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the circulatory and respiratory systems
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
Block 43 View block
  • Health Information / Medical Health Intervention (C239) / Obstetrical and Fetal Interventions / Termination of Pregnancy
  • Health Information / Reproductive history / Number of children
  • Health Information / Reproductive history / Parity/Gravidity
Block 44 View block
  • Health Information / Women's health / Menstruation
Block 45 View block
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Genitourinary System
Block 46 View block
  • Health Information / Medication Intake (ATC/DDD) / Genito urinary system and sex hormones (G)
Block 47 View block
  • Health Information / Medication Intake (ATC/DDD) / Genito urinary system and sex hormones (G)
Block 50 View block
  • Sociodemographic Characteristics / Gender
Block 51 View block
  • Sociodemographic Characteristics / Age/Birth Date / Subject age / birth date
Block 52 View block
  • Body structures / Anthropometric structures / Anthropometric measures / Weight
  • Life Habits/Behaviours / Nutrition / Nutritional Behaviours and Perception of Nutritional Habits
  • Life Habits/Behaviours / Weight related behaviours
Block 53 View block
  • Health Information / Participants Early Life Conditions / Participant's Birth weight
Block 54 View block
  • Health Information / Participants Early Life Conditions / Participant's Birth height
Block 55 View block
  • Health Information / Participants Early Life Conditions / Participant's Pregnancy duration
Block 56 View block
  • Life Habits/Behaviours / Early life/childhood / History of Being Breastfed
Block 57 View block
  • Health Information / Individual Disease history / Congenital malformations, deformations and chromosomal abnormalities
  • Health Information / Participants Early Life Conditions / Participant's Newborn/childhood health
Block 58 View block
  • Life Habits/Behaviours / Tobacco use
Block 59 View block
  • Physical Environment / Environmental exposures / Chemical environmental exposures / Passive Smoke Exposure
  • Physical Environment / Occupational Exposures / Chemical Occupational Exposures / Passive Smoke Exposure
Block 60 View block
  • Physical Environment / Early life and in utero exposures / Chemical Exposures / Passive Smoke Exposure
Block 61 View block
  • Life Habits/Behaviours / Physical activity / Activities/Exercise and Frequency
Block 62 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Social Environment / Familial and social environment
  • Social Environment / Working Social Environment
Block 63 View block
  • Physical Environment / Environmental exposures / Biological environmental exposures
  • Physical Environment / Environmental exposures / Chemical environmental exposures
Block 64 View block
  • Social Environment / Familial and social environment
  • Social Environment / Working Social Environment
Block 67 View block
  • Life Habits/Behaviours / Physical activity / Activities/Exercise and Frequency
Block 68 View block
  • Physical Environment / Early life and in utero exposures / Biological Exposures
  • Physical Environment / Early life and in utero exposures / Chemical Exposures

General information

Name Physical and Cognitive Measures
Last update 07/27/2009
Collected at phase Baseline

Contact

Bruce HR Wolffenbuttel (Scientific Director)
University Medical Center Groningen
Groningen P.O. Box 30001
Phone: +31-50-3613962
Email: bwo@int.umcg.nlMail

Physical and cognitive measures collected

Legend Yes No

BODY STRUCTURES

ANTHROPOMETRIC STRUCTURES

Anthropometric measures

Yes Weight

Yes Height

Yes Standing height

Yes Body circumferences

Yes Waist circumference

Yes Hip circumference

OTHER BODY STRUCTURES

Yes Other body structure measures (Measurement of skin autofluorescence with a non-invasive AGE-reader (Advanced Glycation Endproducts))

BODY FUNCTIONS

MENTAL FUNCTIONS

Global mental functions

Yes Cognitive functions (Ruffs Figure Fluency Test (RFFT))

Yes Other global mental functions (e.g. IQ) (MMSE (minimal mental state examination) for participants of 65 years and older)

Specific mental functions

Yes Other specific mental functions (Neuropsychiatric interview in which a number of basic questions are asked about fears and depression.)

FUNCTIONS OF THE CARDIOVASCULAR AND RESPIRATORY SYSTEMS

Functions of the cardiovascular system

Yes Blood pressure (Using a DINAMAP, measurements during 10 minutes)

Yes Heart rate

Yes Heart rate at rest

Yes Electrical activity (12-lead electrocardiogram)

Functions of the respiratory system

Yes Respiratory functions (Vital Capacity test (VC) and Forced expiratory volume (FEV1 ))

Administration mode

Legend Yes No

Administration environment

Yes Hospital, clinic, university or recruitment center

Yes Participant residence

No Other

Staff administering test

Yes Professionals (nurses, clinicians, etc)

Yes Interviewers/research assistants

No Other

Documentation on procedures

Administration language Dutch

Utilization conditions

SOPs are in Dutch, and describe generally accepted procedures

Standard operating procedures (SOPs) or reference information

General Information

Name DNA Processing
Study name LifeLines Cohort Study & Biobank [LifeLines]
Last update 03/20/2009

Contact

Bruce HR Wolffenbuttel (Scientific Director)
 University Medical Center Groningen
Groningen P.O. Box 30001
Phone: +31-50-3613962
Email: bwo@int.umcg.nlMail

Samples

Legend Yes No
Sample name Blood
  Main laboratory
  Sample labels/traceability (% of samples)
  • No RFID radio frequency identification
  • No Barcode
  • No Printed labels
  • No Handwritten numbers
  • No Others
Pre-extraction sample storage format Substrate storage condition
  • Yes Fresh
  • No Refrigerated
  • Yes Frozen
  • Yes Dried (paper)
  • No Liquid Nitrogen
  • No Other
Substrate storage period
  • No Short term
  • No Long term
DNA extraction DNA extraction method
  • Yes Salting out
  • No Phenol-chloroform
  • No Columnary-extraction
  • No Magnetic beads
  • No Oragene (saliva)
  • No Others
Automation of handling
  • No Manual
  • Yes Automated
DNA handling and storage DNA diluent
  • No TE
  • No H2O
  • No Others
DNA concentration (ng/µl)
Storage conditions
  • Yes 4°C
  • No -20°C
  • No -40°C
  • Yes -80°C
  • No Dried
  • No Others
DNA quantification Methods
  • Yes A260/A280
  • No Picogreen
  • Yes Nanodrop
  • No Others
Technical quality
  • Yes Automated
  • No Manual
  • No Reproducibility
DNA quality control Percentage of samples that are tested
DNA quality method
  • No Agarose gel
  • No PCR
  • No A260/A280 ratio
  • No Others
DNA analysis Platforms
  • No Gentoyping
  • No Sequencing
  • No Methylation
  • No CNVs
  • No CGH
  • No FISH
  • No Other
Technical quality
  • No Automated
  • No Manual
  • No Reproducibility
© 2005 Public Population Project in Genomics.
All rights reserved.
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