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LifeGene [LifeGene] Download as PDF

GENERAL INFORMATION

Name LifeGene
Acronym LifeGene
Last update 04/09/2009

Affiliation

Principal investigators

Jan-Eric Litton (Prof)
Department of Medical epidemiology and biostatistics, Karolinska Institute
Nancy Pedersen (Prof.)
Department of Medical epidemiology and biostatistics, Karolinska Institute

Contacts

Jan-Eric Litton (Prof)
Department of Medical Epidemiology and Biostatistics, Karolinska Institutet
Stockholm
Phone: +46-8 524 87759
Email: Jan-Eric.Litton@ki.seMail
Helena Andersson
Department of Medical epidemiology and biostatistics, Karolinska Institute
Stockholm S-171 77
Phone: +46-8-524 823 72
Email: helena.andersson@ki.seMail

Website

Funding agencies

  • Stockholm County Council
  • Swedish Scientific Council
  • Karolinska Institute

BACKGROUND

LifeGene is a Swedish population-based prospective cohort study on lifestyle, health and genomics. Our intention is, using existing national resources, e.g. the Swedish Twin Registry and Multiple Generation Register, as a springboard, to develop the infrastructure to initiate a large, genetically informative prospective cohort. Because of its prospective design, LifeGene goes several steps further than current collections of samples that may be limited to specific geographical regions of Sweden, specific ascertainment schemes or limited by size.

OBJECTIVES

The ultimate objective of LifeGene health is to improve public health through providing an open resource to researchers. There will be a focus on disease etiology and everyday health problems. Growing health concerns, e.g. obesity, diabetes, allergies, psychiatric disorders, will be addressed. Susceptibility to common infections and the underlying mechanisms for infections being risk factors in development of chronic disease will be studied. Study features will be:
- Ascertaining a cohort of at least ½ million Swedes, potentially starting with all first degree relatives of the twins in the Swedish Twin Registry;
- Repeated assessment of in depth exposure information through electronic means (web, cell-phones, digital paper and TV, etc);
- Repeated surveillance of morbidity through electronic means, including algorithm based assessment of diagnoses for outcomes not typically reported in national health registers, quality registers or ascertained via hospital discharge (e.g. acute infections, psychiatric disorders, allergy, IBS);
- Linkage with all available sources of medical record information (inpatient registry, cancer registry, and when possible, through primary care records);
- Collection of DNA and serum/plasma from all members of the cohort at initiation;
- Collection of other tissues (whole blood, serum/plasma, CSF, skin biopsies, etc.) as specific research questions dictate.

METHODS

Legend Yes No Unknown

General design

Study design Cohort
Type of participants Families
Target or final number of participants 500000
    Target or final number of families No information available
Target or final number of DNA No information available

Participant selection / Characteristics of the population

Selection criteria

Yes Age    Maximun: 55 years
Yes Country of residence Sweden

Recruitment procedures

Initially, enrollment of subjects will be performed by use of national databases such as Swedish Twin Registry, followed by enrollment of subjects through contacts via mass e-mails, advertisements, for enrichment of the cohort with under-studied ethnic populations, etc.

Data Sources

  Cross-sectional Repeated/continuous
Yes Questionnaires to participants/respondents No Yes
Yes Direct physical measures No Yes
Yes Biological samples Yes No
Yes Medical paper
Yes Electronic databases
No Genealogical records  

Collection procedures

Participants will be requested to fill out web-based questionnaires and answer questions by use of cell-phones etc. Data on exposures and phenotypes will be collected repeatedly over time. Linkage to comprehensive registers with health care information will be performed yearly. Blood sampling for DNA extraction and analyses of key biomarkers will be performed according to standardized procedures. Data collection will be for research purpose only. All data will be obtained in coded format. Subject identification data registered in conjunction to biological samples will be stored in such a way that unauthorized persons will have no access and be able to link data and/or samples to the donors. Information transmitted by electronic means will be encrypted. Subject data transferred to a third country (e.g. outside EU) must be de-identified. If data cannot be de-identified before transfer, data security issues must be agreed upon in writing in advance.

Follow up procedures

No information available

Baseline principal variables of interest

Health information

  • Yes Diseases history - ICD-10 All conditions
  • Yes Familial disease history
  • Yes Early life
  • Yes Women's health
  • Yes Quality of life

Physical / Biochemical measures

Yes Body structure measures Yes Biochemical measures
Yes Body function measures  

Sociodemographic Characteristics

Yes Birth location Yes Parents birth location
Yes Ethnicity/Race Yes Gender
Yes Marital status Yes Date of birth

Socioeconomic Characteristics

Yes Education level No Income
Yes Working status  

Life habits / Behaviours

Yes Physical activity Yes Smoking / Tobacco Use
Yes Nutrition Yes Alcohol intake

Physical environment

Yes Passive smoking Yes Mobile phone use

Sample management

Biological samples

Yes Blood Yes Buccal cells
No Cord blood No Tissues
Unknown Saliva Unknown Urine

Biological samples format

  Fresh Frozen Dried (paper) Other Specification
Blood No Yes No No
Buccal cells No Yes   No

GOVERNANCE

Legend Yes No

Allow access to data or samples to external researchers

Unknown Data (questionnaire-derived, measured…)
Unknown Biological samples

STATUS

Current status

Phase Start End
Preparation phase/Pilot 2009 2009
Recruitment / initial data collection 2010 --
Follow-up of participants -- --
Current number of participants recruited 0
Current number of collected DNA samples --

AVAILABLE DOCUMENTS

  Document type Availability
1 General study protocol
2 Specific protocols/procedures (data collection, samples management, etc.)
3 Questionnaires
4 Physical and cognitive measures
5 DNA processing
6 Consent forms
7 Pamphlet / project description
8 Governance / ethics documentation
9 Other

General Information

Name Adult and Child Baseline
Last update 11/06/2009
Version or reference year version 0.8
Phase Baseline
Supplementary informations Currently under development

Questionnaire owner

  • LifeGene

Contact

Mikael Eriksson
Medical Epidemiology and Biostatistics, Karolinska Institutet
Solna
Phone: +46 8 524 823 58
Email: mikael.eriksson@ki.seMail

Utilization conditions

Some instruments are proprietary, e.g. DAWBA

Methods

Legend Yes No

Respondent

  • Yes Participant
  • No Proxy

Administration environment

  • Yes Over the phone
  • No Hospital, clinic, university or recruitment center
  • No Respondent / Proxy residence
  • Yes Other (Web)

Administration mode

  • Yes Auto administered
  • No Auto administrated with face to face validation by trained personnel
  • No Administrated by trained personel / Physician

Administration format

  • No Paper questionnaire
  • Yes Computerized

Administration language

  • English
  • Swedish

Documents

Available Format

NOTICE: HTML document format may differ slightly from the actual questionnaire. Please refer to the original PDF document for exact reproduction.

General Information

Name Adult and Child Baseline
Number of Question Blocks 77

Questionnaire and Question Block List

Click on the View block to see the block of questions related to a domain or a group of domains.
Block 1 View block
  • Socioeconomic Characteristics / Income
Block 2 View block
  • Sociodemographic Characteristics / Household Status / Structure of the Household
  • Sociodemographic Characteristics / Household Status / Subjet Household Status
Block 3 View block
  • Health Information / Reproductive history / Number of children
Block 4 View block
  • Socioeconomic Characteristics / Education level / Subject education level
Block 5 View block
  • Socioeconomic Characteristics / Working status / Subject Working status/history
Block 6 View block
  • Physical Environment / Occupational Exposures / Chemical Occupational Exposures / Others
  • Physical Environment / Occupational Exposures / Physical Occupational Exposures / Noise, vibrations, etc.
Block 7 View block
  • Social Environment / Working Social Environment
Block 8 View block
  • Health Information / Perception of health/quality of life
  • Life Habits/Behaviours / Physical activity / Perception of physical health
Block 9 View block
  • Health Information / Medication Intake (ATC/DDD)
Block 10 View block
  • Health Information / Perception of health/quality of life
Block 11 View block
  • Health Information / Perception of health/quality of life
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Life Habits/Behaviours / Physical activity / Activities/Exercise and Frequency
  • Life Habits/Behaviours / Travel History and Habits
Block 12 View block
  • Health Information / Individual Disease history / Certain infectious and parasitic diseases
  • Life Habits/Behaviours / Sexual behaviours
Block 13 View block
  • Physical Environment / Environmental exposures / Physical environmental exposures / Radiation (ionizing and non ionizing)
Block 14 View block
  • Health Information / Medication Intake (ATC/DDD) / Various
  • Life Habits/Behaviours / Nutrition / Food intake and Frequency / Salt and Food Supplement
Block 15 View block
  • Life Habits/Behaviours / Physical activity / Activities/Exercise and Frequency
Block 16 View block
  • Health Information / Women's health / Menopause
  • Health Information / Women's health / Menstruation
Block 17 View block
  • Health Information / Individual Disease history / Diseases of the genitourinary system
  • Health Information / Individual Disease history / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
  • Health Information / Medication Intake (ATC/DDD) / Genito urinary system and sex hormones (G)
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
Block 19 View block
  • Health Information / Reproductive history / Parity/Gravidity
Block 20 View block
  • Health Information / Medical Health Intervention (C239) / Obstetrical and Fetal Interventions / Anteparturn Obstetrical Interventions
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions / Therapeutic Interventions on the Genitourinary System
  • Health Information / Medical Health Intervention (C239) / Physical/Physiological Therapeutic Interventions
Block 21 View block
  • Health Information / Individual Disease history / Diseases of the genitourinary system
Block 22 View block
  • Health Information / Medication Intake (ATC/DDD) / Genito urinary system and sex hormones (G)
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the circulatory and respiratory systems
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the skin and subcutaneous tissue
  • Health Information / Women's health / Menopause
  • Life Habits/Behaviours / Sleep Behaviours
Block 23 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the urinary system
Block 24 View block
  • Health Information / Medical Health Intervention (C239) / Therapeutic Interventions Strengthening the Immune System and/or Genetic Composition
  • Health Information / Medication Intake (ATC/DDD) / Antiinfectives for systemic use (J)
Block 25 View block
  • Health Information / Individual Disease history / Diseases of the musculoskeletal system and connective tissue
Block 26 View block
  • Health Information / Familial Disease History (ICD10*) / Diseases of the musculoskeletal system and connective tissue
  • Health Information / Individual Disease history / Diseases of the musculoskeletal system and connective tissue
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
  • Health Information / Medication Intake (ATC/DDD) / Cardiovascular system (C)
  • Health Information / Medication Intake (ATC/DDD) / Dermatologicals (D)
  • Health Information / Medication Intake (ATC/DDD) / Respiratory system (R)
  • Health Information / Medication Intake (ATC/DDD) / Sensory organs (S)
  • Health Information / Medication Intake (ATC/DDD) / Systemic hormonal preparations, excl. Sex hormones and insulins (H)
  • Health Information / Women's health / Menopause
Block 27 View block
  • Life Habits/Behaviours / Tobacco use
Block 28 View block
  • Physical Environment / Environmental exposures / Chemical environmental exposures / Passive Smoke Exposure
  • Social Environment / Familial and social environment
Block 29 View block
  • Life Habits/Behaviours / Alcohol use
  • Social Environment / Familial and social environment
Block 30 View block
  • Life Habits/Behaviours / Illicit drug use
Block 31 View block
  • Health Information / Medical Health Intervention (C239) / Other Healthcare Interventions
Block 32 View block
  • Health Information / Individual Disease history / Diseases of the digestive system
Block 33 View block
  • Health Information / Individual Disease history / Certain infectious and parasitic diseases
  • Health Information / Individual Disease history / Diseases of the circulatory system
  • Health Information / Individual Disease history / Diseases of the digestive system
  • Health Information / Individual Disease history / Diseases of the genitourinary system
  • Health Information / Individual Disease history / Diseases of the respiratory system
  • Health Information / Individual Disease history / Diseases of the skin and subcutaneous tissue
  • Health Information / Individual Disease history / Endocrine, nutritional and metabolic diseases
  • Health Information / Individual Disease history / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
Block 34 View block
  • Health Information / Individual Disease history / Diseases of the circulatory system
  • Health Information / Medication Intake (ATC/DDD) / Blood and blood forming organs (B)
Block 35 View block
  • Health Information / Individual Disease history / Endocrine, nutritional and metabolic diseases
Block 36 View block
  • Health Information / Individual Disease history / Diseases of the digestive system
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
Block 37 View block
  • Health Information / Individual Disease history / Diseases of the genitourinary system
Block 38 View block
  • Health Information / Individual Disease history / Diseases of the digestive system
Block 39 View block
  • Health Information / Individual Disease history / Diseases of the respiratory system
Block 40 View block
  • Health Information / Individual Disease history / Diseases of the skin and subcutaneous tissue
Block 41 View block
  • Health Information / Individual Disease history / Endocrine, nutritional and metabolic diseases
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
Block 42 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
Block 43 View block
  • Health Information / Individual Disease history / Certain infectious and parasitic diseases
  • Health Information / Individual Disease history / Diseases of the eye and adnexa
  • Health Information / Individual Disease history / Diseases of the musculoskeletal system and connective tissue
  • Health Information / Individual Disease history / Diseases of the nervous system
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Individual Disease history / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving speech and voice
Block 44 View block
  • Health Information / Individual Disease history / Diseases of the nervous system
Block 45 View block
  • Health Information / Individual Disease history / Diseases of the musculoskeletal system and connective tissue
  • Health Information / Medication Intake (ATC/DDD) / Musculo-skeletal system (M)
Block 46 View block
  • Health Information / Individual Disease history / Diseases of the nervous system
Block 47 View block
  • Health Information / Individual Disease history / Mental and behavioural disorders
Block 48 View block
  • Body structures / Anthropometric structures / Anthropometric measures / Height
Block 49 View block
  • Body structures / Anthropometric structures / Anthropometric measures / Weight
Block 50 View block
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
  • Life Habits/Behaviours / Nutrition / Nutritional Behaviours and Perception of Nutritional Habits
  • Life Habits/Behaviours / Physical activity / Activities/Exercise and Frequency
Block 51 View block
  • Body structures / Anthropometric structures / Anthropometric measures / Height
  • Body structures / Anthropometric structures / Anthropometric measures / Weight
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Health Information / Women's health / Menstruation
  • Life Habits/Behaviours / Nutrition / Nutritional Behaviours and Perception of Nutritional Habits
  • Life Habits/Behaviours / Weight related behaviours
Block 52 View block
  • Health Information / Individual Disease history / Diseases of the digestive system
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
Block 53 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
Block 54 View block
  • Life Habits/Behaviours / Sleep Behaviours
Block 55 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
Block 56 View block
  • Health Information / Individual Disease history / Certain infectious and parasitic diseases
  • Health Information / Individual Disease history / Diseases of the ear and mastoid process
  • Health Information / Medical Health Intervention (C239) / Cognitive, Psychosocial and Sensory Therapeutic Interventions / Therapeutic Interventions for Hearing
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
Block 57 View block
  • Health Information / Individual Disease history / Injury, poisoning and certain other consequences of external causes
Block 58 View block
  • Physical Environment / Environmental exposures / Biological environmental exposures / Others
Block 59 View block
  • Physical Environment / Environmental exposures / Chemical environmental exposures / Others
Block 60 View block
  • Life Habits/Behaviours / Tobacco use
Block 61 View block
  • Health Information / Individual Disease history / Diseases of the respiratory system
  • Health Information / Medical Health Intervention (C239) / Other Healthcare Interventions
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
  • Health Information / Medication Intake (ATC/DDD) / Dermatologicals (D)
  • Health Information / Medication Intake (ATC/DDD) / Respiratory system (R)
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the circulatory and respiratory systems
Block 62 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the circulatory and respiratory systems
Block 63 View block
  • Health Information / Individual Disease history / Diseases of the blood and blood-forming organs and certain disorders involving the immune mechanism
  • Health Information / Medication Intake (ATC/DDD) / Alimentary tract and metabolism (A)
  • Health Information / Medication Intake (ATC/DDD) / Dermatologicals (D)
  • Health Information / Medication Intake (ATC/DDD) / Respiratory system (R)
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the circulatory and respiratory systems
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the digestive system and abdomen
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the skin and subcutaneous tissue
Block 64 View block
Block 65 View block
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
Block 66 View block
  • Health Information / Individual Disease history / External causes of morbidity and mortality
  • Health Information / Individual Disease history / Factors influencing health status and contact with health services
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Medical Health Intervention (C239) / Other Healthcare Interventions
Block 67 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
Block 68 View block
  • Health Information / Individual Disease history
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
Block 69 View block
  • Health Information / Individual Disease history / Factors influencing health status and contact with health services
Block 70 View block
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
Block 71 View block
  • Health Information / Individual Disease history / Mental and behavioural disorders
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
Block 72 View block
  • Sociodemographic Characteristics / Kinship
Block 73 View block
  • Health Information / Perception of health/quality of life
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
Block 74 View block
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving the nervous and musculoskeletal systems
Block 75 View block
  • Health Information / Perception of health/quality of life
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / Symptoms and signs involving cognition, perception, emotional state and behaviour
Block 76 View block
  • Health Information / Medical Health Intervention (C239) / Other Healthcare Interventions
Block 77 View block
  • Life Habits/Behaviours / Tobacco use
Block 78 View block
  • Health Information / Individual Disease history / Diseases of the blood and blood-forming organs and certain disorders involving the immune mechanism
  • Health Information / Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified / General symptoms and signs

General information

Name Physical and cognitive measures
Last update 06/12/2009
Collected at phase Baseline
Year interval 2010 to 2016

Contacts

Nancy Pedersen (Prof.)
Department of Medical Epidemiology and Biostatistics, Karolinska Institutet
Stockholm
Phone: +46 8 524 87418
Email: Nancy.Pedersen@ki.seMail

Physical and cognitive measures collected

Legend Yes No

BODY STRUCTURES

ANTHROPOMETRIC STRUCTURES

Anthropometric measures

Yes Weight

Yes Height

Yes Standing height

Yes Body circumferences

Yes Waist circumference

Yes Hip circumference

Body composition

Yes Bioimpedance

BODY FUNCTIONS

SENSORY FUNCTIONS AND PAIN

Hearing and vestibular functions

Yes Hearing

FUNCTIONS OF THE CARDIOVASCULAR AND RESPIRATORY SYSTEMS

Functions of the cardiovascular system

Yes Blood pressure

Yes Heart rate

Yes Heart rate at rest

Functions of the respiratory system

Yes Respiratory functions (VC and FVC)

Administration mode

Legend Yes No

Administration environment

Yes Hospital, clinic, university or recruitment center

No Participant residence

No Other

Staff administering test

Yes Professionals (nurses, clinicians, etc)

No Interviewers/research assistants

No Other

Documentation on procedures

Administration language Swedish

General Information

Name DNA Processing
Study name LifeGene [LifeGene]
Last update 05/13/2008

Contacts

Camilla Lagerberg (M. Sc)
 Stockholm
Phone: +46-8-52482375
Email: Camilla.Lagerberg@ki.seMail
Gunnel Tybring (PhD)
 Stockholm SE 171 77
Phone: +46 8 524 83640
Email: gunnel.tybring@ki.seMail

Samples

Legend Yes No
Sample name Blood Buccal cells Saliva
  Main laboratory Karolinska Institutet Biobank Karolinska Institutet Biobank Karolinska Institutet Biobank
  Sample labels/traceability (% of samples)
  • No RFID radio frequency identification
  • No Barcode
  • No Printed labels
  • No Handwritten numbers
  • No Others
  • No RFID radio frequency identification
  • No Barcode
  • No Printed labels
  • No Handwritten numbers
  • No Others
  • No RFID radio frequency identification
  • No Barcode
  • No Printed labels
  • No Handwritten numbers
  • No Others
Pre-extraction sample storage format Substrate storage condition
  • Yes Fresh
  • No Refrigerated
  • Yes Frozen
  • Yes Dried (paper) (filter paper)
  • No Liquid Nitrogen
  • No Other
  • Yes Fresh
  • No Refrigerated
  • Yes Frozen
  • No Dried (paper)
  • No Liquid Nitrogen
  • No Other
  • Yes Fresh
  • No Refrigerated
  • Yes Frozen
  • No Dried (paper)
  • No Liquid Nitrogen
  • No Other
Substrate storage period
  • No Short term
  • No Long term
  • No Short term
  • No Long term
  • No Short term
  • No Long term
DNA extraction DNA extraction method
  • Yes Salting out
  • No Phenol-chloroform
  • No Columnary-extraction
  • No Magnetic beads
  • No Oragene (saliva)
  • No Others
  • Yes Salting out
  • No Phenol-chloroform
  • No Columnary-extraction
  • No Magnetic beads
  • No Oragene (saliva)
  • No Others
  • Yes Salting out
  • No Phenol-chloroform
  • No Columnary-extraction
  • No Magnetic beads
  • No Oragene (saliva)
  • No Others
Automation of handling
  • No Manual
  • Yes Automated
  • No Manual
  • Yes Automated
  • No Manual
  • Yes Automated
Protocols Autopure LS robot from Gentra systems Autopure LS robot from Gentra systems Autopure LS robot from Gentra systems
DNA handling and storage DNA diluent
  • Yes TE
  • No H2O
  • No Others
  • Yes TE
  • No H2O
  • No Others
  • Yes TE
  • No H2O
  • No Others
DNA concentration (ng/µl)
Storage conditions
  • No 4°C
  • No -20°C
  • No -40°C
  • Yes -80°C
  • No Dried
  • No Others
  • No 4°C
  • No -20°C
  • No -40°C
  • Yes -80°C
  • No Dried
  • No Others
  • No 4°C
  • No -20°C
  • No -40°C
  • Yes -80°C
  • No Dried
  • No Others
DNA quantification Methods
  • Yes A260/A280
  • No Picogreen
  • No Nanodrop
  • No Others
  • Yes A260/A280
  • No Picogreen
  • No Nanodrop
  • No Others
  • Yes A260/A280
  • No Picogreen
  • No Nanodrop
  • No Others
Technical quality
  • No Automated
  • Yes Manual
  • No Reproducibility
  • No Automated
  • Yes Manual
  • No Reproducibility
  • No Automated
  • Yes Manual
  • No Reproducibility
DNA quality control Percentage of samples that are tested
DNA quality method
  • No Agarose gel
  • No PCR
  • Yes A260/A280 ratio
  • No Others
  • No Agarose gel
  • No PCR
  • Yes A260/A280 ratio
  • No Others
  • No Agarose gel
  • No PCR
  • Yes A260/A280 ratio
  • No Others
Protocols Step one would be to further evaluate the QC harmonization program. We have an ISO accreditation for all processes in our Biobank Step one would be to further evaluate the QC harmonization program. We have an ISO accreditation for all processes in our Biobank Step one would be to further evaluate the QC harmonization program. We have an ISO accreditation for all processes in our Biobank
DNA analysis Platforms
  • No Gentoyping
  • No Sequencing
  • No Methylation
  • No CNVs
  • No CGH
  • No FISH
  • No Other
  • No Gentoyping
  • No Sequencing
  • No Methylation
  • No CNVs
  • No CGH
  • No FISH
  • No Other
  • No Gentoyping
  • No Sequencing
  • No Methylation
  • No CNVs
  • No CGH
  • No FISH
  • No Other
Technical quality
  • No Automated
  • No Manual
  • No Reproducibility
  • No Automated
  • No Manual
  • No Reproducibility
  • No Automated
  • No Manual
  • No Reproducibility

General Information

Name Information Technologies
Study name LifeGene [LifeGene]
Last update 04/16/2009

Contact

Mats Thorman
Department of Medical epidemiology and biostatistics, Karolinska Institute
Stockholm SE 171 77
Phone: +46 8 524 823 22
Email: mats.thorman@ki.seMail

Software

Legend Yes No

Software overview

Biobanking areas covered LabWare LIMS version 5 LifeGene Central Repository Confirmit Professional version 14 Episerver CMS 5 Microsoft Dynamics CRM 4.0
Participant recruitment (ex: CATI, CRM) Yes
Data collection Yes Yes
Sample collection, storage and processing Yes
Data repository Yes
Other Yes
 
Programming language LabWare LIMS version 5 LifeGene Central Repository Confirmit Professional version 14 Episerver CMS 5 Microsoft Dynamics CRM 4.0
Name and version of programming language LIMS Basic .Net 3.5 ASP .Net 3.5
 
Internal/external development LabWare LIMS version 5 LifeGene Central Repository Confirmit Professional version 14 Episerver CMS 5 Microsoft Dynamics CRM 4.0
In-house by IT department
By an external company Yes Yes Yes Yes Yes
 
Shareability LabWare LIMS version 5 LifeGene Central Repository Confirmit Professional version 14 Episerver CMS 5 Microsoft Dynamics CRM 4.0
Freely available
Purchaseable Yes Yes Yes Yes
Needs extensive work Yes
Not for share

Software details

Biobanking areas covered

  • No Participant recruitment (ex: CATI, CRM)
  • Yes Data collection
  • No Questionnaires (ex: CAPI)
  • Yes Physical and cognitive measures
  • Yes Sample collection, storage and processing
  • Yes Collection
  • Yes Sample bio-repository management system (ex: sample storage management software)
  • Yes LIMS (ex: biochemical analysis, DNA extraction, genotyping)
  • No Data repository
  • No Data warehousing
  • No Study monitoring (ex: quality control)
  • No User data / sample request management

Name and version of programming language

LIMS Basic

Internal/external development

  • No In-house by IT department
  • Yes By an external company (LabWare)

Shareability

  • No Freely available
  • Yes Purchaseable
  • No Needs extensive work
  • No Not for share

Biobanking areas covered

  • No Participant recruitment (ex: CATI, CRM)
  • No Data collection
  • No Questionnaires (ex: CAPI)
  • No Physical and cognitive measures
  • No Sample collection, storage and processing
  • No Collection
  • No Sample bio-repository management system (ex: sample storage management software)
  • No LIMS (ex: biochemical analysis, DNA extraction, genotyping)
  • Yes Data repository
  • Yes Data warehousing
  • Yes Study monitoring (ex: quality control)
  • Yes User data / sample request management

Name and version of programming language

.Net 3.5

Internal/external development

  • No In-house by IT department
  • Yes By an external company (Silobreaker)

Shareability

  • No Freely available
  • No Purchaseable
  • Yes Needs extensive work
  • No Not for share

Supplementary informations

The central repository stores all collected data about participants in order to handle data request and to facilitate exchange of the other systems (LIMS, Confirmit etc).

Biobanking areas covered

  • No Participant recruitment (ex: CATI, CRM)
  • Yes Data collection
  • Yes Questionnaires (ex: CAPI)
  • No Physical and cognitive measures
  • No Sample collection, storage and processing
  • No Collection
  • No Sample bio-repository management system (ex: sample storage management software)
  • No LIMS (ex: biochemical analysis, DNA extraction, genotyping)
  • No Data repository
  • No Data warehousing
  • No Study monitoring (ex: quality control)
  • No User data / sample request management

Internal/external development

  • No In-house by IT department
  • Yes By an external company (Confirmit)

Shareability

  • No Freely available
  • Yes Purchaseable
  • No Needs extensive work
  • No Not for share

Biobanking areas covered

  • No Participant recruitment (ex: CATI, CRM)
  • No Data collection
  • No Questionnaires (ex: CAPI)
  • No Physical and cognitive measures
  • No Sample collection, storage and processing
  • No Collection
  • No Sample bio-repository management system (ex: sample storage management software)
  • No LIMS (ex: biochemical analysis, DNA extraction, genotyping)
  • No Data repository
  • No Data warehousing
  • No Study monitoring (ex: quality control)
  • No User data / sample request management
  • Yes Other (Primary source for information and interaction with participants in the study.)

Name and version of programming language

ASP .Net 3.5

Internal/external development

  • No In-house by IT department
  • Yes By an external company (EPiSERVER)

Shareability

  • No Freely available
  • Yes Purchaseable
  • No Needs extensive work
  • No Not for share

Biobanking areas covered

  • Yes Participant recruitment (ex: CATI, CRM)
  • No Data collection
  • No Questionnaires (ex: CAPI)
  • No Physical and cognitive measures
  • No Sample collection, storage and processing
  • No Collection
  • No Sample bio-repository management system (ex: sample storage management software)
  • No LIMS (ex: biochemical analysis, DNA extraction, genotyping)
  • No Data repository
  • No Data warehousing
  • No Study monitoring (ex: quality control)
  • No User data / sample request management

Internal/external development

  • No In-house by IT department
  • Yes By an external company (Microsoft)

Shareability

  • No Freely available
  • Yes Purchaseable
  • No Needs extensive work
  • No Not for share

Comments

No information available

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